Salivary Gland Gene Expression Search Guide

1. The search term can be Gene Symbol, Gene Description or Gene Ontology Term as shown on the search page.
2. The developmental time courses of SMG and SLGs and regions analyzed by laser capture microdissection (LCM) experiments are shown in the two explanatory images.
3. If a single gene matches the search term, the individual expression page is displayed. If more genes match, a list of genes with mini expression patterns is presented. Some genes do not have all three patterns due to experimental differences. Each gene symbol links to the expression pattern page.
4. The signal intensity range is around 10 to 500,000 arbitrary units. We recommend caution when the maximum signal in a pattern is below 100. The signal/noise ratio might be low. Data should always be confirmed by PCR.
5. On the expression page, SMG, SLG and SMG LCM patterns are displayed. In addition, related Gene Ontology terms and pathways, if they exist, are displayed and linked to geneontology.org and kegg.jp respectively.
6. On the expression page, the Similar Profile link leads to a list of other genes that have similar expression patterns as generated by the Kmeans clustering method.
7. On the expression page, the Probe Pattern link leads to raw expression patterns of microarray probes of that gene. The Agilent platform has multiple probes for some genes and some probes work better than others, giving higher expression values. The probe expression pattern helps to evaluate the gene expression pattern.
8. On the top of the expression page, links to Entrez, Pubmed and GenBank are provided.
9. On the Similar Profile page, gene names in red share more similar patterns than those in blue. Each name is linked to the expression page.
10. At the bottom of the Similar Profile page, the list is displayed. The list can be copied and pasted to other analysis computing applications. The link to MsigDB is provided and the list can be analyzed there.